| Article ID | Journal | Published Year | Pages | File Type |
|---|---|---|---|---|
| 2089373 | Journal of Immunological Methods | 2006 | 5 Pages |
Abstract
We describe a semi-quantitative method for measuring the relative affinity of antigen-specific oligoclonal IgG bands separated by isoelectric focusing followed by blotting onto antigen-coated membrane and incubation with sodium thiocyanate. When the developed blot is digitised in greyscale, densitograms can be made and peak areas calculated using ImageJ freeware. By expressing peak area as a percentage of the total area under the curve we have shown that there is a statistically significant rise in percentage of peak area for a given band which persists with increasing molarities of sodium thiocyanate.
Keywords
Related Topics
Life Sciences
Biochemistry, Genetics and Molecular Biology
Biotechnology
Authors
Miles D. Chapman, Geoffrey Keir, Axel Petzold, Edward J. Thompson,