| Article ID | Journal | Published Year | Pages | File Type |
|---|---|---|---|---|
| 2089714 | Journal of Microbiological Methods | 2016 | 4 Pages |
Abstract
•The auto-fluorescence of cyanobacteria interferes with FISH experiments.•Chemical treatments with H2O2 or CuSO4 before mounting reduce the auto-fluorescence.•These protocol were tested and optimized on unicellular Microcystis strains.•We successfully applied this protocol in FISH imaging of filamentous cyanobacteria.
Cyanobacteria contain pigments, which generate auto-fluorescence that interferes with fluorescence in situ hybridization (FISH) imaging of cyanobacteria. We describe simple chemical treatments using CuSO4 or H2O2 that significantly reduce the auto-fluorescence of Microcystis strains. These protocols were successfully applied in FISH experiments using 16S rRNA specific probes and filamentous cyanobacteria.
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Authors
Perrine Zeller, Olivier Ploux, Annick Méjean,