Article ID | Journal | Published Year | Pages | File Type |
---|---|---|---|---|
2092144 | Microbiological Research | 2015 | 6 Pages |
Abstract
Lysozyme (1,4-β-N-acetylmuramidase) is commonly applied in the food, medical, and pharmaceutical industries. In this study, we tested a novel application of fluorescein-modified lysozyme (using carboxyfluorescein with a triazine-based coupling reagent) as a new tool for the detection of Gram-positive soil bacteria. The results, obtained by cultivation methods, fluorescence analysis, and laser interferometry, showed that, after optimization, fluorescein-modified lysozyme could be used to evaluate the prevalence of Gram-positive bacteria essential in bioremediation of soils with low pH, such as those degraded by sulfur.
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Authors
Michał Arabski, Iwona Konieczna, Ewa Tusińska, Sławomir Wąsik, Inga Relich, Krzysztof Zając, Zbigniew J. Kamiński, Wiesław Kaca,