Comparison of the SYBR Green and the hybridization probe format for real-time PCR detection of HHV-6

SummaryA comparative study was conducted of a novel real-time quantitative PCR test (LightCycler System) with FastStart DNA MasterPLUS SYBR Green I dye to detect DNA of human herpes virus 6 (HHV-6). Results were compared with those of a real-time quantitative PCR with hybridization probe (HP) formats using the fluorescence resonance energy transfer method, and with those of a single qualitative PCR test. The detection limit of the test with SYBR Green I dye was 20 copies of the virus, similar to that of the other two tests. The reproducibility was satisfactory. The new test has the same advantages as real-time PCR with HP formats and offers a greater versatility at lower cost.