Article ID | Journal | Published Year | Pages | File Type |
---|---|---|---|---|
2093574 | Stem Cell Reports | 2015 | 14 Pages |
•PSA-NCAM− cells isolated from neural rosettes are classified as multipotent NCSCs•NCSCs are a potential target for tumor prevention in hPSC-derived-NPC-based therapy•Removal of PSA-NCAM− cells prevents the introduction of mesodermal tumor in the CNS•Removal of PSA-NCAM− cells prevents the introduction of unwanted grafts in the CNS
SummaryTumorigenic potential of human pluripotent stem cells (hPSCs) is an important issue in clinical applications. Despite many efforts, PSC-derived neural precursor cells (NPCs) have repeatedly induced tumors in animal models even though pluripotent cells were not detected. We found that polysialic acid-neural cell adhesion molecule (PSA-NCAM)− cells among the early NPCs caused tumors, whereas PSA-NCAM+ cells were nontumorigenic. Molecular profiling, global gene analysis, and multilineage differentiation of PSA-NCAM− cells confirm that they are multipotent neural crest stem cells (NCSCs) that could differentiate into both ectodermal and mesodermal lineages. Transplantation of PSA-NCAM− cells in a gradient manner mixed with PSA-NCAM+ cells proportionally increased mesodermal tumor formation and unwanted grafts such as PERIPHERIN+ cells or pigmented cells in the rat brain. Therefore, we suggest that NCSCs are a critical target for tumor prevention in hPSC-derived NPCs, and removal of PSA-NCAM− cells eliminates the tumorigenic potential originating from NCSCs after transplantation.
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