Article ID | Journal | Published Year | Pages | File Type |
---|---|---|---|---|
2093717 | Stem Cell Reports | 2015 | 13 Pages |
•Longitudinal monitoring of murine mesoangioblasts with BLI and small-animal PET•Noninvasive evaluation of immune suppressant efficacy•Inhibition of co-stimulation outperformed cyclosporin•Inhibition of co-stimulation reduced cytotoxic and upregulated regulatory T cells
SummaryMuscular dystrophies are a heterogeneous group of myopathies, characterized by muscle weakness and degeneration, without curative treatment. Mesoangioblasts (MABs) have been proposed as a potential regenerative therapy. To improve our understanding of the in vivo behavior of MABs and the effect of different immunosuppressive therapies, like cyclosporine A or co-stimulation-adhesion blockade therapy, on cell survival noninvasive cell monitoring is required. Therefore, cells were transduced with a lentiviral vector encoding firefly luciferase (Fluc) and the human sodium iodide transporter (hNIS) to allow cell monitoring via bioluminescence imaging (BLI) and small-animal positron emission tomography (PET). Non-H2 matched mMABs were injected in the femoral artery of dystrophic mice and were clearly visible via small-animal PET and BLI. Based on noninvasive imaging data, we were able to show that co-stim was clearly superior to CsA in reducing cell rejection and this was mediated via a reduction in cytotoxic T cells and upregulation of regulatory T cells.