| Article ID | Journal | Published Year | Pages | File Type |
|---|---|---|---|---|
| 2093781 | Stem Cell Reports | 2015 | 16 Pages |
•Applied quantitative iTRAQ mass spectrometry to analyze stem cell populations•Defined the cell-surface proteome of human mesenchymal progenitor cells•Validated ten cell-surface proteins as mesenchymal progenitor biomarkers•Showed their loss-of-function effects on mesenchymal progenitor cells
SummaryMesenchymal progenitor cells have great therapeutic potential, yet incomplete characterization of their cell-surface interface limits their clinical exploitation. We have employed subcellular fractionation with quantitative discovery proteomics to define the cell-surface interface proteome of human bone marrow mesenchymal stromal/stem cells (MSCs) and human umbilical cord perivascular cells (HUCPVCs). We compared cell-surface-enriched fractions from MSCs and HUCPVCs (three donors each) with adult mesenchymal fibroblasts using eight-channel isobaric-tagging mass spectrometry, yielding relative quantification on >6,000 proteins with high confidence. This approach identified 186 upregulated mesenchymal progenitor biomarkers. Validation of 10 of these markers, including ROR2, EPHA2, and PLXNA2, confirmed upregulated expression in mesenchymal progenitor populations and distinct roles in progenitor cell proliferation, migration, and differentiation. Our approach has delivered a cell-surface proteome repository that now enables improved selection and characterization of human mesenchymal progenitor populations.
