| Article ID | Journal | Published Year | Pages | File Type |
|---|---|---|---|---|
| 2107198 | Cancer Cell | 2013 | 15 Pages |
•The EMT-TF reprogramming predicts poor outcome in melanoma patients•The EMT-TF reprogramming is dependent on ERK activity and involves FRA1•ZEB1 and TWIST1 are oncogenic; ZEB2 and SNAIL2 are oncosuppressive in melanoma•The antagonistic properties of EMT-TFs mirror their opposing effects on MITF
SummaryAberrant expression of embryonic epithelial-mesenchymal transition-inducing transcription factors (EMT-TFs) in epithelial cells triggers EMT, neoplastic transformation, stemness, and metastatic dissemination. We found that regulation and functions of EMT-TFs are different in malignant melanoma. SNAIL2 and ZEB2 transcription factors are expressed in normal melanocytes and behave as tumor-suppressor proteins by activating an MITF-dependent melanocyte differentiation program. In response to NRAS/BRAF activation, EMT-TF network undergoes a profound reorganization in favor of TWIST1 and ZEB1. This reversible switch cooperates with BRAF in promoting dedifferentiation and neoplastic transformation of melanocytes. We detected EMT-TF reprogramming in late-stage melanoma in association with enhanced phospho-ERK levels. This switch results in E-cadherin loss, enhanced invasion, and constitutes an independent factor of poor prognosis in melanoma patients.
