Article ID Journal Published Year Pages File Type
2107521 Cancer Cell 2010 13 Pages PDF
Abstract

SummaryRegulators of mitosis have been successfully targeted to enhance response to taxane chemotherapy. Here, we show that the salt inducible kinase 2 (SIK2) localizes at the centrosome, plays a key role in the initiation of mitosis, and regulates the localization of the centrosome linker protein, C-Nap1, through S2392 phosphorylation. Interference with the known SIK2 inhibitor PKA induced SIK2-dependent centrosome splitting in interphase while SIK2 depletion blocked centrosome separation in mitosis, sensitizing ovarian cancers to paclitaxel in culture and in xenografts. Depletion of SIK2 also delayed G1/S transition and reduced AKT phosphorylation. Higher expression of SIK2 significantly correlated with poor survival in patients with high-grade serous ovarian cancers. We believe these data identify SIK2 as a plausible target for therapy in ovarian cancers.

► A kinome screen identifies SIK2 as a centrosome kinase required for mitosis ► SIK2 phosphorylates C-Nap1 and is required for centrosome separation in mitosis ► Targeted SIK2 depletion results in synergy with taxanes in ovarian cancers ► Depletion of SIK2 results in delayed G1/S transition and low AKT phosphorylation

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Life Sciences Biochemistry, Genetics and Molecular Biology Cancer Research
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