Article ID | Journal | Published Year | Pages | File Type |
---|---|---|---|---|
2110432 | Cancer Genetics | 2013 | 7 Pages |
Abstract
To better define the place of multiplex ligation-dependent probe amplification (MLPA) in routine cytogenetic diagnosis in chronic lymphocytic leukemia (CLL), we compared MLPA and fluorescence in situ hybridization (iFISH) data obtained in 77 CLL patients. Although MLPA detected most recurrent copy number genomic aberrations (90.9%), false-negative results were found in cases with small-size abnormal clones and false-positive MLPA findings resulting from point mutations (TP53) or an apparent lack of probe specificity (chromosome 19) were observed. Thus, MLPA may be a useful complementary but not alternative approach for iFISH testing of genomic aberration in CLL.
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Authors
Lauren Véronèse, Olivier Tournilhac, Patricia Combes, Nolwen Prie, Eléonore Pierre-Eymard, Romain Guièze, Richard Veyrat-Masson, Jacques-Olivier Bay, Philippe Vago, Andreï Tchirkov,