| Article ID | Journal | Published Year | Pages | File Type |
|---|---|---|---|---|
| 2112709 | Cancer Letters | 2014 | 8 Pages |
•The modified classical SERPA technique could be used for the discovery of tumor-associated antigens and autoantibodies.•We have examined the presence of antibodies against HSP105 in sera from ESCC for the first time.•Autoantibodies against TIM in ESCC serum mainly reacted with glycosylated but not deglycosylated TIM.•Various expression patterns of HSP105 and TIM were observed in normal epithelium and ESCC.•Autoantibodies against HSP105 and TIM may be potentially useful for the early detection of ESCC.
Our aim was to identify novel tumor-associated antigens from the esophageal squamous cell carcinoma (ESCC) cell line EC0156, and related autoantibodies in sera from patients with ESCC. We used modified serological proteome analysis, involving one- and two-dimensional electrophoresis, Western blot, and MALDI-TOF/TOF-MS to identify 6 ESCC-associated antigens. From these, 105 kDa heat shock protein (HSP105) and triosephosphate isomerase (TIM) were further evaluated and we determined they could induce autoantibody responses in ESCC sera and are highly expressed in ESCC tissues. Anti-HSP105 and anti-TIM autoantibodies were found in 39.1% (18/46) and 34.8% (16/46) of patients with ESCC, respectively, but only in two controls. A receiver operating characteristic curve constructed with HSP105 and TIM gave a sensitivity of 54.3% and 95% (38/40) specificity in discriminating ESCC from matched controls. Interestingly, we found that autoantibodies against TIM in ESCC serum mainly reacted with glycosylated but not deglycosylated TIM. The preliminary results suggest the potential utility of screening autoantibodies in sera for use as biomarkers for cancer diagnosis.
