Article ID Journal Published Year Pages File Type
2113202 Cancer Letters 2012 9 Pages PDF
Abstract

The E6 oncoprotein of human papillomavirus (HPV) is critical in cervical cancer development. Using the yeast two-hybrid assay, we showed that HPV-16 E6 (16E6) interacts with one of the DNA fragmentation factors (DFFs), DFF40, which mediates DNA degradation during apoptosis. Furthermore, 16E6 interacts with DFF40 through its zinc finger motif 2 and a bridge section linking the two zinc finger motifs. DNA fragmentation assays disclosed that 16E6 binding to DFF40 leads to blockage of DNA cleavage. Our data collectively suggest that suppression of DNA fragmentation through 16E6–DFF40 interaction is a central event promoting tumorigenesis.

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Life Sciences Biochemistry, Genetics and Molecular Biology Cancer Research
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