Adoptive immunotherapy of cancer using ex vivo expanded human γδ T cells: A new approach

γδ T cells can be an option for adoptive immunotherapy of cancer. The major obstacle to clinical application of γδ T cells is their low number and lack of a reliable method to expand them consistently and efficiently.We were able to expand γδ T cells with high purity in all donors regardless of their starting repertoire of γδ T cells. These ex vivo expanded γδ T cells are in early differentiation stage, can efficiently kill various tumors and inhibit growth of human lung cancer xenografts.This new approach for ex vivo expansion of human γδ T cells will open new horizons for clinical use of these cells.