Article ID | Journal | Published Year | Pages | File Type |
---|---|---|---|---|
2115291 | Cancer Letters | 2007 | 10 Pages |
Abstract
Ellipticine induces formation of two DNA adducts in leukemia HL-60 and CCRF-CEM cells, identical with deoxyguanosine adducts generated by ellipticine metabolites 13-hydroxyellipticine and 12-hydroxyellipticine in vitro and in vivo. The ellipticine cytotoxicity to HL-60 (IC50 = 0.64 μM) and CCRF-CEM cells (IC50 = 4.7 μM) correlates with levels of DNA adducts. The different expressions of enzymes activating ellipticine in cells explain this finding. While cytochrome P450 1A1 and cyclooxygenase-1 are expressed in both cells, HL-60 cells express also high levels of another activator, myeloperoxidase. The results suggest the adduct formation as a new mode of antitumor action of ellipticine for leukemia.
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Authors
Jitka Poljaková, Eva Frei, Jacobo E. Gomez, Dagmar Aimová, Tomáš Eckschlager, Jan Hraběta, Marie Stiborová,