Article ID | Journal | Published Year | Pages | File Type |
---|---|---|---|---|
2115312 | Cancer Letters | 2007 | 9 Pages |
Abstract
We investigated the Akt-mTOR pathway and effects of rapamycin using human colorectal cancer cell lines. LoVo and CaRI reduced proliferative activity in response to rapamycin in a dose-dependent manner. The phosphorylation of Akt and p70 S6K was prominent in these cells. Rapamycin quickly downregulated phospho-S6K but not phospho-Akt. Therefore, phospho-S6K is considered a good indicator of the activated Akt-mTOR pathway as well as rapamycin sensitivity in colorectal cancer cells. By immunohistochemical study, nearly 40% of adenomas and carcinomas of the colorectum exhibited either partial or whole positive staining for phospho-S6K, suggestive of rapamycin-sensitive lesions.
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Authors
Hiroaki Nozawa, Toshiaki Watanabe, Hirokazu Nagawa,