Article ID | Journal | Published Year | Pages | File Type |
---|---|---|---|---|
2115935 | Cancer Letters | 2006 | 9 Pages |
Abstract
We have established two murine cell lines derived from Small Cell Lung Carcinomas (SCLCs) developed by HPV-E6/E7 transgenic mice. These cells named PPAP-9 and PPAP-10 were isolated from mice bearing tumors, 9 and 10 months old, respectively. The cells, 5 μm in diameter, express HPV oncoproteins and sustain tumor formation after subcutaneous injection in syngenic mice. A detailed analysis indicated the epithelial origin and the neuroendocrine differentiation of these cells. We showed by confocal immunofluorescence the expression of the epithelial marker cytokeratin 5, whose gene promoter was used to direct the expression of HPV E6/E. Cells express several neuroendocrine markers such as CGRP, MAP-2, Ash1, CgrA, Scg2. The neuroendocrine differentiation of these cells was further confirmed by electron microscopy demonstrating neuropeptides secreting granules in their cytoplasm. Furthermore, in agreement with the altered expression observed in the majority of human SCLC we showed in these cells the absence of both p53 and pRB and a dramatic reduction in the expression of Caveolin-1.
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Cancer Research
Authors
Laura Carraresi, Rosanna Martinelli, Alessandro Vannoni, Massimo Riccio, Maja Dembic, Sergio Tripodi, Marcella Cintorino, Spartaco Santi, Elisa Bigliardi, Mario Carmellini, Mara Rossini,