Differentiation of cancer cell type and phenotype using quantum dot-gold nanoparticle sensor arrays

We demonstrate rapid and efficient sensing of mammalian cell types and states using nanoparticle-based sensor arrays. These arrays are comprised of cationic quantum dots (QDs) and gold nanoparticles (AuNPs) that interact with cell surfaces to generate distinguishable fluorescence responses based on cell surface signatures. The use of QDs as the recognition elements as well as the signal transducers presents the potential for direct visualization of selective cell surface interactions. Notably, this sensor is unbiased, precluding the requirement of pre-knowledge of cell state biomarkers and thus providing a general approach for phenotypic profiling of cell states, with additional potential for imaging applications.

► A rapid and efficient sensor for mammalian cell types and states using nanoparticle-based sensor arrays is presented. ► The sensing approach relies on selective interactions between nanoparticles and cell surfaces. ► This unbiased array-based sensor possesses the potential of cancer detectionanddiagnostic imaging.