Article ID | Journal | Published Year | Pages | File Type |
---|---|---|---|---|
2116469 | Cancer Letters | 2010 | 9 Pages |
Abstract
The in vitro chemosensitizing effect of triptolide (PG490) on the cytotoxicity of 5-fluorouracil (5-FU) was determined in three colorectal cancer (CRC) cell lines. PG490 alone was toxic to CRC cell lines (IC50s: 1.39–5.51 nM). The cytotoxicity of 5-FU to CRC cell lines was 4–15-fold sensitized by combining use of low concentration of PG490. CI-isobologram indicated that the effect of PG490 plus 5-FU was synergistic. PG490 and 5-FU treatment induced activated caspase 3 and Bax expression and inhibited Bcl-2 expression. PG490 simultaneously induced generation of reactive oxygen species (ROS) and inhibited NF-κB transcriptional activity.
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Authors
Bing Xu, Xiaoxia Guo, Sumi Mathew, Angel L Armesilla, James Cassidy, John L Darling, Weiguang Wang,