The Human Glioblastoma Cell Culture Resource: Validated Cell Models Representing All Molecular Subtypes

•The HGCC resource contains 48 annotated human GBM cell lines and an interactive database•The GBM cell lines are propagated in stem cell conditions and display GSC characteristics•The HGCC resource provides cell lines of all molecular (TCGA) subtypes•All data connected with the HGCC cell lines can be accessed at hgcc.se

Glioblastoma (GBM) is the most frequent and malignant form of primary brain tumor. GBM is essentially incurable and its resistance to therapy is attributed to a subpopulation of cells called glioma stem cells (GSCs). To meet the present shortage of relevant GBM cell (GC) lines we developed a library of annotated and validated cell lines derived from surgical samples of GBM patients, maintained under conditions to preserve GSC characteristics. This collection, which we call the Human Glioblastoma Cell Culture (HGCC) resource, consists of a biobank of 48 GC lines and an associated database containing high-resolution molecular data. We demonstrate that the HGCC lines are tumorigenic, harbor genomic lesions characteristic of GBMs, and represent all four transcriptional subtypes. The HGCC panel provides an open resource for in vitro and in vivo modeling of a large part of GBM diversity useful to both basic and translational GBM research.