Article ID | Journal | Published Year | Pages | File Type |
---|---|---|---|---|
2124425 | European Journal of Cancer | 2008 | 8 Pages |
Abstract
We investigated the correlation between the response to fluoropyrimidines as first-line therapy and the expressions of genes in patients with primary colorectal cancer (CRC). The study group comprised 92 patients with metastatic CRC. Total RNA was isolated from laser-captured tumour cells in surgically resected primary lesions, and gene expression was quantitatively evaluated by real-time RT-PCR assay. Low thymidylate synthase (TS), low γ-glutamyl hydrolase, high reduced folate carrier 1, high O6-methylguanine-DNA methyltransferase (MGMT) and low cyclin E expressions were associated with a good response (P = 0.0030, 0.0250, 0.0120, 0.0030 and 0.0020, respectively) on univariate analysis. On multivariate logistic regression analysis, TS and MGMT remained independent predictors of the response. The clinical response rates were 63.2% in the low TS or high MGMT group and 14.3% in high TS and low MGMT group (P < 0.0001). The combination of high TS and low MGMT expression is a significant predictor of a poor response to fluoropyrimidine treatment.
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Authors
Takako Eguchi Nakajima, Yasuhide Yamada, Tadakazu Shimoda, Junichi Matsubara, Ken Kato, Tetsuya Hamaguchi, Yasuhiro Shimada, Yoshihiro Okayama, Toshinori Oka, Kuniaki Shirao,