Article ID | Journal | Published Year | Pages | File Type |
---|---|---|---|---|
2130977 | Experimental Cell Research | 2011 | 10 Pages |
Abstract
Dimeric intercellular adhesion molecule-1 (ICAM-1) has been known to more efficiently mediate cell adhesion than monomeric ICAM-1. Here, we found that truncation of the intracellular domain of ICAM-1 significantly enhances surface dimerization based on the two criteria: 1) the binding degree of monomer-specific antibody CA-7 and 2) the ratio of dimer/monomer when a mutation (L42 â C42) was introduced in the interface of domain 1. Mutation analysis revealed that the positively charged amino acids, including very membrane-proximal 505R, are essential for maintaining the structural transition between the monomer and dimer. Despite a strong dimer presentation, the ICAM-1 mutants lacking an intracellular domain (IC1ÎCTD) or containing R to A substitution in position 505 (505R/A) supported a lower degree of cell adhesion than did wild-type ICAM-1. Collectively, these results demonstrate that the native structure of surface ICAM-1 is not a dimer, but is an intermediate monomer-dimer equilibrium structure by which the effectiveness of ICAM-1 can be fully achieved.
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Authors
Hyun-Mee Oh, Min-Sung Kwon, Hyang-Jin Kim, Byeong-Hun Jeon, Hye-Ran Kim, Hyang-Ok Choi, Bo-Ra Na, Soo-Hyun Eom, Nam Woong Song, Chang-Duk Jun,