Article ID | Journal | Published Year | Pages | File Type |
---|---|---|---|---|
2131976 | Experimental Cell Research | 2010 | 13 Pages |
Abstract
Microtubules are part of cell structures that play a role in regulating the migration of cancer cells. The cellular apoptosis susceptibility (CSE1L/CAS) protein is a microtubule-associated protein that is highly expressed in cancer. We report here that CSE1L regulates the association of α-tubulin with β-tubulin and promotes the migration of MCF-7 breast cancer cells. CSE1L was associated with α-tubulin and β-tubulin in GST (glutathione S-transferase) pull-down and immunoprecipitation assays. CSE1L-GFP (green fluorescence protein) fusion protein experiments showed that the N-terminal of CSE1L interacted with microtubules. Increased CSE1L expression resulted in decreased tyrosine phosphorylation of α-tubulin and β-tubulin, increased α-tubulin and β-tubulin association, and enhanced assembly of microtubules. Cell protrusions or pseudopodia are temporary extensions of the plasma membrane and are implicated in cancer cell migration and invasion. Increased CSE1L expression increased the extension of MCF-7 cell protrusions. In vitro migration assay showed that enhanced CSE1L expression increased the migration of MCF-7 cells. Our results indicate that CSE1L plays a role in regulating the extension of cell protrusions and promotes the migration of cancer cells.
Related Topics
Life Sciences
Biochemistry, Genetics and Molecular Biology
Cancer Research
Authors
Cheng-Jeng Tai, Shing-Chuan Shen, Woan-Ruoh Lee, Ching-Fong Liao, Win-Ping Deng, Hung-Yi Chiou, Cheng-I Hsieh, Jai-Nien Tung, Ching-Shyang Chen, Jeng-Fong Chiou, Li-Tzu Li, Chuang-Yu Lin, Chung-Huei Hsu, Ming-Chung Jiang,