Article ID | Journal | Published Year | Pages | File Type |
---|---|---|---|---|
2132078 | Experimental Cell Research | 2010 | 16 Pages |
Abstract
The mechanisms of secretory transport through the Golgi apparatus remain an issue of debate. The precise functional importance of calcium ions (Ca2+) for intra-Golgi transport has also been poorly studied. Here, using different approaches to measure free Ca2+ concentrations in the cell cytosol ([Ca2+]cyt) and inside the lumen of the Golgi apparatus ([Ca2+]GA), we have revealed transient increases in [Ca2+]cyt during the late phase of intra-Golgi transport that are concomitant with a decline in the maximal [Ca2+]GA restoration ability. Thus, this redistribution of Ca2+ from the Golgi apparatus into the cytosol during the movement of cargo through the Golgi apparatus appears to have a role in intra-Golgi transport, and mainly in the late Ca2+-dependent phase of SNARE-regulated fusion of Golgi compartments.
Keywords
VSVGFluo-3Procollagen Isoluble N-ethylmaleimide-sensitive factor attachment protein receptorsARFcoat protein IHEPES-buffered salt solutionFluo-3-AMCHXHFSSNAREHBSSFura-2Fura-2-AM[Ca2+]cytFluorescence resonance energy transferFRETAequorinGolgi apparatuscycloheximideendoplasmic reticulumADP-ribosylation factorHuman fibroblastsCalciumCOPIgalactosyl transferase
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Authors
Massimo Micaroni, Giuseppe Perinetti, Daniele Di Giandomenico, Katiuscia Bianchi, Alexander Spaar, Alexander A. Mironov,