Article ID Journal Published Year Pages File Type
2132570 Experimental Cell Research 2009 9 Pages PDF
Abstract

To investigate nuclear lamina re-assembly in vivo, Drosophila A-type and B-type lamins were artificially expressed in Drosophila lamin Dm0null mutant brain cells. Both exogenous lamin C (A-type) and Dm0 (B-type) formed sub-layers at the nuclear periphery, and efficiently reverted the abnormal clustering of the NPC. Lamin C initially appeared where NPCs were clustered, and subsequently extended along the nuclear periphery accompanied by the recovery of the regular distribution of NPCs. In contrast, lamin Dm0 did not show association with the clustered NPCs during lamina formation and NPC spacing recovered only after completion of a closed lamin Dm0 layer. Further, when lamin Dm0 and C were both expressed, they did not co-polymerize, initiating layer formation in separate regions. Thus, A and B-type lamins reveal differing properties during lamina assembly, with A-type having the primary role in organizing NPC distribution. This previously unknown complexity in the assembly of the nuclear lamina could be the basis for intricate nuclear envelope functions.

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