Article ID | Journal | Published Year | Pages | File Type |
---|---|---|---|---|
2136334 | Leukemia Research | 2016 | 9 Pages |
Abstract
Multiple myeloma (MM) is caused by proliferation of clonal plasma cells (cPCs) in bone marrow (BM), associated with numerical and functional defects in immune subsets. An impairment of B cell compartment is involved in onset/progression of the disease. Methods: By flow cytometry, we studied distribution of naïve/transitional (IgD+CD27â), memory unswitched (IgD+CD27+), memory switched (IgDâCD27+) and double negative (DN) (IgDâCD27â) B lymphocytes in BM of control subjects, and responding and relapsing patients. Results: We observed an increased percentage of IgD+CD27+ B cells in healthy controls vs responding patients (p < 0.05). Treated non complete responders exhibited an expanded DN compartment vs stringent complete responders (p = 0.011); in turn IgD+CD27â subpopulation was larger in stringent complete responders vs other responding patients (p = 0.006). None of the studied B cell subsets showed clonal restriction. Correlation analysis revealed negative correlations between naïve/transitional and DN B cells in all groups, except in newly diagnosed subjects. Conclusions: This may be considered a feasible start point to explore the importance of B cells in the immunosuppressive MM BM microenvironment, correlating these findings with immunosenescence and therapy related increased risk of infection. Moreover, we propose a possible role of naïve/transitional and DN B cells as predictive markers in treated patients.
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Authors
Fanny Pojero, Alessandra Casuccio, Caterina Giambanco, Matteo Bulati, Silvio Buffa, Francesco Di Bassiano, Francesco Gervasi, Calogero Caruso, Giuseppina Colonna Romano,