Article ID | Journal | Published Year | Pages | File Type |
---|---|---|---|---|
2137171 | Leukemia Research | 2012 | 5 Pages |
Abstract
Erythroid differentiation is a tightly regulated multi-step process that has not been fully elucidated. We previously reported that a siRNA screened from random siRNA library, siRNA clone-67, induced erythroid differentiation in human erythroleukemia K-562 cell line. Here we identified that human CCDC12 (coiled-coil domain containing 12) is a target of siRNA clone-67, by target capture with biotinylated siRNA. Over-expression of CCDC12 in K-562 cell up-regulated the expression of CD235, É-globin and γ-globin, accelerated cell growth, and slightly down-regulated the expression of GATA-2. Knockdown of CCDC12 slowed down the cell growth. These data indicate that CCDC12 is a new participant that promotes early erythroid differentiation.
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Authors
Cuiqing Fan, Lin Dong, Ning Zhu, Yuan Xiong, Jiewen Zhang, Le Wang, Yan Shen, Xiaoyin Zhang, Meihong Chen,