Article ID | Journal | Published Year | Pages | File Type |
---|---|---|---|---|
2137551 | Leukemia Research | 2010 | 6 Pages |
We demonstrate herein the augmentation of rituximab-mediated apoptosis in lymphoma cell lines by cross-linking with recombinant FcγRs, which is further enhanced by using a nonfucosylated variant of rituximab having strong FcγRIII-binding capacity. Furthermore, we show that neutrophils can serve as physiological cross-linkers that augment anti-CD20-mediated apoptosis, as evidenced by (i) the neutrophil-augmented apoptosis was more profound for the nonfucosylated variant of rituximab and (ii) the mechanism depended on FcγRIIIb but not on FcγRIIa. Taken together, we suggest a potential anti-tumour mechanism of nonfucosylated anti-CD20 antibody by which antibody molecules are cross-linked through enhanced interaction with FcγRIIIb in neutrophils.