Article ID | Journal | Published Year | Pages | File Type |
---|---|---|---|---|
2138061 | Leukemia Research | 2011 | 6 Pages |
Abstract
BCR-ABL1 kinase domain mutations were evaluated in 60 imatinib-resistant patients with Philadelphia-positive (Ph+) leukemia using PCR-Invader assay and direct sequencing. In chronic myelogenous leukemia (CML) – chronic phase (CP), 5 had P-loop mutations and 3 had T315I mutations. CML-CP patients with high Sokal score showed significantly higher incidence of mutations. P-loop mutations were associated with higher risk of disease progression. In CML-advanced phase, P-loop mutations and T315I mutation were associated with significantly shorter survival. In Ph+ acute lymphoblastic leukemia, overall survival was poor irrespective of mutational status. The PCR-Invader assay is useful for screening of mutations and prediction of prognosis.
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Authors
Takaaki Ono, Shuichi Miyawaki, Fumihiko Kimura, Heiwa Kanamori, Shigeki Ohtake, Kunio Kitamura, Hiroyuki Fujita, Isamu Sugiura, Kensuke Usuki, Nobuhiko Emi, Shigehisa Tamaki, Yasutaka Aoyama, Hiroyasu Kaya, Tomoki Naoe, Kenichi Tadokoro,