Article ID | Journal | Published Year | Pages | File Type |
---|---|---|---|---|
2138270 | Leukemia Research | 2010 | 9 Pages |
Abstract
The t(8;21) Acute Myeloid Leukaemia (AML) Kasumi-1 cell line with N822K KIT mutation, is a model system for leukemogenesis. As AML initiating cells reside in the CD34+CD38− fraction, we addressed the refined cytogenomic characterization and miRNA expression of Kasumi-1 cell line and its FACS-sorted subpopulations focussing on this compartment. By conventional cytogenetics, Spectral-Karyotyping and array-CGH the cytogenomic profile of Kasumi-1 cells evidenced only subtle regions differentially represented in CD34+CD38− cells.Expression profiling by a miRNA platform showed a set of miRNA differentially expressed in paired subpopulations and the signature of miR-584 and miR-182 upregulation in the CD34+CD38− fraction.
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Authors
Laura Pedranzini, Federica Mottadelli, Simona Ronzoni, Franca Rossella, Manuela Ferracin, Ivana Magnani, Gaia Roversi, Patrizia Colapietro, Massimo Negrini, Pier Giuseppe Pelicci, Lidia Larizza,