Article ID | Journal | Published Year | Pages | File Type |
---|---|---|---|---|
2138987 | Leukemia Research | 2006 | 8 Pages |
Abstract
A humanized monoclonal antibody (mAb) against HM1.24 (AHM) caused antibody-dependent cellular cytotoxicity (ADCC) against multiple myeloma (MM) cells. Here, we constructed a conventional non-radioisotope method that quantifies the amount of HM1.24 using fluorescein-labeled AHM. More than 104 molecules/cell of HM1.24 were detected in 12 out of 14 patients’ MM cells, and a linear correlation was found between ADCC by AHM and the amounts of HM1.24. Thus, AHM is likely to be more efficacious against MM cells with high levels of HM1.24. This conventional non-RI method to quantify HM1.24 will be useful to select patients most likely to respond to AHM.
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Authors
Shigeto Kawai, Yasuo Koishihara, Shin-ichiro Iida, Shuji Ozaki, Toshio Matsumoto, Masaaki Kosaka, Hisafumi Yamada-Okabe,