Article ID | Journal | Published Year | Pages | File Type |
---|---|---|---|---|
2139407 | Leukemia Research | 2007 | 11 Pages |
Abstract
Tumor cells with different origins have different threshold to apoptosis. Hematopoietic (Jurkat, NCI-H929) cells and non-hematopoietic (A549, MCF-7) cells were received hyperbaric oxygen (HBO2) treatment from 2.5 to 3.5 atmosphere absolute (ATA) of 100% oxygen for 6 h, and a significant percentage of apoptosis were shown only in hematopoietic Jurkat and NCI-H929 cells by either Annexin V or TUNEL assay. Oxidative stress was illustrated higher in HBO2-treated hematopoietic cells by superoxide fluorochrome detectors. HBO2 treatment leads to caspase-3, caspase-7 activation and further cleavage of PARP within cells. Furthermore, the increased phosphorylation of p38 MAPK was demonstrated in both Jurkat and NCI-H929 cells.
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Authors
Yen-Chen Chen, Shao-Yuan Chen, Pey-Shen Ho, Chia-Huei Lin, Ya-Yuan Cheng, Jehng-Kang Wang, Huey-Kang Sytwu,