| Article ID | Journal | Published Year | Pages | File Type |
|---|---|---|---|---|
| 2140147 | Leukemia Research | 2007 | 8 Pages |
Abstract
Genistein (GEN) is a natural protein tyrosine kinase inhibitor. We analyzed the molecular response of HL-60 cells to GEN treatment by gel-based proteomics approach. Fourteen differentially expressed proteins which are functionally involved in metabolism, cell signaling, RNA processing, cell proliferation and motility, and chaperones were identified. Both the dose- and time-dependent up-regulation of Hsp70 protein 8 and heterogeneous nuclear ribonucleoprotein (hnRNP) H1, and the down-regulation of Rab14, hnRNP C and stathmin-1 by GEN were verified by immunoblot analysis. Our novel findings provide insightful clues to the potential therapeutic targets for leukemia treatment in diverse tyrosine kinase-dependent cellular pathways.
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Authors
Daohai Zhang, Yan-Chin Tai, Ching-Ho Stephen Wong, Lee-Kian Tai, Evelyn Siew-Chuan Koay, Chien-Shing Chen,