| Article ID | Journal | Published Year | Pages | File Type |
|---|---|---|---|---|
| 2146535 | Mutation Research/Fundamental and Molecular Mechanisms of Mutagenesis | 2012 | 8 Pages |
Recent studies indicated that there is a direct link between central carbon metabolism (CCM) and initiation and elongation of DNA replication in Eschericha coli. Namely, effects of certain mutations in genes coding for replication proteins (dnaA, dnaB, dnaE, dnaG, and dnaN) could be specifically suppressed by deletions of some genes, whose products are involved in CCM reactions (pta, ackA, pgi, tktB, and gpmA). Here, we demonstrate that the link between CCM and DNA synthesis can be extended to the DNA replication fidelity, as we report changes of the mutator phenotypes of E. coli dnaQ49 and dnaX36 mutants (either suppression or enhancement) by dysfunctions of zwf, pta, ackA, acnB, and icdA genes. Overexpression of appropriate wild-type CCM genes in double mutants resulted in reversions to the initial mutator phenotypes, indicating that the effects were specific. Moreover, the observed suppression and enhancement effects were not caused by changes in bacterial growth rates. These results suggest that there is a genetic correlation between CCM and DNA replication fidelity in E. coli, apart from the already documented link between CCM and DNA replication initiation control and elongation efficiency.
► Mutants in some CCM genes changed effects of dnaQ49 and dnaX36 alleles in Escherichia coli. ► These changes were reversed by expression of wild-type CCM genes in trans. ► The changes in mutator phenotypes were not caused by different cell growth rates. ► There is a genetic link between CCM and DNA replication fidelity in E. coli.
