| Article ID | Journal | Published Year | Pages | File Type |
|---|---|---|---|---|
| 2146591 | Mutation Research/Fundamental and Molecular Mechanisms of Mutagenesis | 2011 | 8 Pages |
Abstract
⺠Use comet assay to examine diepoxybutane-induced DNA crosslinks and breaks in cells. ⺠Diepoxybutane induces DNA-DNA rather than DNA-protein crosslinks in L02 cells. ⺠200 μM is a threshold for the formation of crosslinks in large quantities. ⺠Diepoxybutane induces both single-strand breaks and alkali-labile sites.
Keywords
MMSDNA–protein cross-linksRCEDiepoxybutane1,2,3,4-diepoxybutanetBHPSSBDPCICLEBDDEBGAPDHDSBDMEMBDDFBS1,3-Butadiene3-(4,5-dimethyl-2-thiazolyl)-2,5-diphenyl-2H-tetrazolium bromide3-Butene-1,2-diol3,4-epoxy-1-buteneInterstrand cross-linksDMSODulbecco's modified Eagle's mediumMTTALSDimethyl sulfoxideComet assayalkali-labile sitesfetal bovine serumsingle-strand breaksdouble-strand breaksFormaldehydemethyl methanesulfonateAGTtert-butyl hydroperoxidePropidium iodideProteinase Kglyceraldehydes 3-phosphate dehydrogenase
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Authors
Ying Wen, Pan-Pan Zhang, Jing An, Ying-Xin Yu, Ming-Hong Wu, Guo-Ying Sheng, Jia-Mo Fu, Xin-Yu Zhang,