| Article ID | Journal | Published Year | Pages | File Type |
|---|---|---|---|---|
| 2147230 | Mutation Research/Fundamental and Molecular Mechanisms of Mutagenesis | 2008 | 5 Pages |
Abstract
The induction and disappearance of DNA double strand breaks (DSBs) after irradiation of G1 and mitotic cells were compared with the γ-H2AX foci assay and a gel electrophoresis assay. This is to determine whether cell cycle related changes in chromatin structure might influence the γ-H2AX assay which depends on extensive phosphorylation and dephosphorylation of the H2AX histone variant surrounding DSBs. The disappearance of γ-H2AX foci after irradiation was much slower for mitotic than for G1 cells. On the other hand, no difference was seen for the gel electrophoresis assay. Our data may suggest the limited accessibility of dephosphorylation enzyme in irradiated metaphase cells or trapped γ-H2AX in condensed chromatin.
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Authors
Takamitsu A. Kato, Ryuichi Okayasu, Joel S. Bedford,