Article ID Journal Published Year Pages File Type
2147986 Mutation Research/Genetic Toxicology and Environmental Mutagenesis 2014 4 Pages PDF
Abstract

•Representing γ-rays-induced adaptation in mESCs for first time.•Modification and optimization of cytokinesis-block micronucleus assay for adaptive response in mESCs.•Interpretation of MTT test for adaptation response in mESCs.

Low doses of ionizing radiation may induce an adaptive mechanism which protects embryonic stem cells against higher doses, a phenomenon which was reported previously for somatic cells. In this study, a possible adaptive response (AR) was evaluated by measuring cell survival (MTT assay) and chromosomal aberrations (micronucleus assay). Thymidine-synchronized mouse embryonic stem cells (mESCs) were exposed to 2.5, 3.7, or 5 cGy 60Co γ-rays and, after 5 h challenged by a dose of 150 cGy. mESCs pre-irradiated at 2.5 cGy showed an adaptive response.

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