| Article ID | Journal | Published Year | Pages | File Type |
|---|---|---|---|---|
| 2148074 | Mutation Research/Genetic Toxicology and Environmental Mutagenesis | 2013 | 6 Pages |
The role of different DNA-repair genes (OGG1, XRCC1, XRCC2 and XRCC3) on both the spontaneous and the induced frequency of micronuclei (MN) has been studied in the lymphocytes of a group of 114 patients with differentiated thyroid cancer (DTC). Induction of MN was achieved by treatment of the lymphocytes with 0.5 Gy of gamma-radiation.The selected genes are involved in base-excision repair (BER) (OGG1, Ser326Cys; XRCC1, Arg280His and Arg399Gln), and in homologous recombination repair (HRR) (XRCC2, Arg188His and XRCC3, IVS5-14G). Genotyping was carried out by use of the iPLEX (Sequenom) technique.Results indicate that only the OGG1-Ser326Cys polymorphism was able to modulate the MN frequency. This effect was only observed in the spontaneous MN frequency (P = 0.016), but not in the MN frequency induced after irradiation. In addition, a strong correlation was observed between spontaneous and induced MN frequency, which would suggest an underlying genetic background.
► The potential role of different repair genes in the levels of DNA damage has been evaluated. ► The selected genes have been OGG1, XRCC1, XRCC2 and XRCC3, involved in base excision and in homologous recombination repair pathways. ► Results show that the Ser326Cys OGG1 polymorphism modulate the basal frequency of BNMN. ► No effects were observed for the other genes. ► No interactions were observed between the genes involved in the same pathway.
