Article ID | Journal | Published Year | Pages | File Type |
---|---|---|---|---|
2148166 | Mutation Research/Genetic Toxicology and Environmental Mutagenesis | 2012 | 5 Pages |
Abstract
The comet assay is one of the most widely used methods to evaluate DNA damage and repair in eukaryotic cells. The comets can be measured by software, in a semi-automatic or automatic process. In this paper, we apply the CellProfiler open-source software for automatic analysis of comets from digitized images, reporting the percentage of tail DNA. A side-by-side comparison of CellProfiler with CASP software demonstrated good agreement between the two packages. Our work demonstrates that automatic measurement of silver-stained comets with open-source software is possible, providing significant time savings.
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Authors
J.E. González, I. Romero, J.F. Barquinero, O. García,