| Article ID | Journal | Published Year | Pages | File Type |
|---|---|---|---|---|
| 2148230 | Mutation Research/Genetic Toxicology and Environmental Mutagenesis | 2012 | 12 Pages |
Abstract
⺠Cell transformation assays (CTA) are used to detect in vitro rodent carcinogens. ⺠We present the results on the ECVAM interlaboratory prevalidation study on SHE CTA pH 7.0. ⺠The SHE CTA at pH 7.0 is reproducible within- and between-laboratories. ⺠This protocol and the assay system itself are transferable between laboratories.
Keywords
FBSpH 7.0CTASOPDRFRelative plating efficiencyVMTOECDdose range findingRPEECVAMDRPMtFDMSOSHEcell transformation assaySyrian hamster embryoDimethylsulfoxideAlternative methodstandard operating procedureOrganisation for Economic Cooperation and Developmentfoetal bovine serumSyrian hamster embryo cellsEuropean Centre for the Validation of Alternative MethodsInter-laboratory comparisonplating efficiency
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Authors
Marie-Aline Maire, Kamala Pant, Albrecht Poth, Karl-Rainer Schwind, Claudine Rast, Shannon W. Bruce, Jamie E. Sly, Susanne Kunz-Bohnenberger, Thorsten Kunkelmann, Günter Engelhardt, Markus Schulz, Paule Vasseur,