| Article ID | Journal | Published Year | Pages | File Type |
|---|---|---|---|---|
| 2148796 | Mutation Research/Genetic Toxicology and Environmental Mutagenesis | 2009 | 6 Pages |
The aim of this study was to investigate the cytotoxic and genotoxic effect of copper extracts obtained from metallic copper in Chinese hamster ovary (CHO-K1) cell line using neutral red (NR), sister chromatid exchange (SCE), chromosomal aberrations (CA) and cell-cycle kinetics tests. Cells were cultured in Ham-F10 with different copper-containing extracts obtained after the immersion of copper disks for 1, 2, 3, 9, 12, 24, 48 and 72 h in culture medium.Results from cytotoxicity assay showed an inverted U-shape response evidenced in changes in lysosomal activity and mitotic index. The analysis of CA revealed an increase of abnormal metaphases for copper concentration (cCu) in the 5.67–7.42 mg/L dose-range (p < 0.001). In addition, SCE frequencies were higher for treated cells when compared with controls in the 1.56–7.42 mg/L concentration range (p < 0.001). The absence of metaphases indicated cytotoxicity for cCu ≥ 10.85 mg/L. Results show that cells close to copper-containing materials releasing copper ions are susceptible to cytotoxic and genotoxic effects.
