Article ID | Journal | Published Year | Pages | File Type |
---|---|---|---|---|
2151998 | Neoplasia | 2009 | 14 Pages |
Abstract
Hodgkin lymphoma (HL) is derived from preapoptotic germinal center B cells, although a general loss of B cell phenotype is noted. Using quantitative reverse transcription-polymerase chain reaction and miRNA microarray, we determined the microRNA (miRNA) profile of HL and compared this with the profile of a panel of B-cell non-Hodgkin lymphomas. The two methods showed a strong correlation for the detection of miRNA expression levels. The HL-specific miRNA included miR-17-92 cluster members, miR-16, miR-21, miR-24, and miR-155. Using a large panel of cell lines, we found differential expression between HL and other B-cell lymphoma-derived cell lines for 27 miRNA. A significant down-regulation in HL compared to non-Hodgkin lymphoma was observed only for miR-150. Next, we performed target gene validation of predicted target genes for miR-155, which is highly expressed in HL and is differentially expressed between HL and Burkitt lymphoma. Using luciferase reporter assays, we validated 11 predicted miR-155 target genes in three different HL cell lines. We demonstrated that AGTR1, FGF7, ZNF537, ZIC3, and IKBKE are true miR-155 target genes in HL.
Keywords
PMBLqRT-PCRChlHRSPrimary mediastinal B-cell lymphomaNLPHLDLBCLCLLEBVrenilla luciferaseBurkitt lymphomaDiffuse large B-cell lymphomaHodgkin lymphomaNodular lymphocyte predominant Hodgkin lymphomaClassic Hodgkin lymphomaChronic lymphocytic leukemiaFirefly luciferaseGerminal centerUTR یا untranslated regions untranslated regionMicroRNAMiRNAquantitative reverse transcription-polymerase chain reaction
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Authors
Johan H. Gibcus, Lu Ping Tan, Geert Harms, Rikst Nynke Schakel, Debora de Jong, Tjasso Blokzijl, Peter Möller, Sibrand Poppema, Bart-Jan Kroesen, Anke van den Berg,