| Article ID | Journal | Published Year | Pages | File Type |
|---|---|---|---|---|
| 2167208 | Cellular Immunology | 2012 | 8 Pages |
Recent imaging studies on intact lymph nodes (LNs) of naïve T cell receptor (TCR)-transgenic mice have reported that T cells reduce their motility upon contact with relevant antigen-presenting cells (APCs). Using in vivo two-photon imaging of T cells in joint-draining (JD) LNs, we examined whether similar changes in T cell motility are observed in wild type mice. Co-transfer of T cells from naïve mice and antigen-experienced T cells from mice with proteoglycan (PG)-induced arthritis into naïve or arthritic recipients resulted in prolonged interactions of antigen-experienced T cells with APCs upon intra-articular antigen (PG) injection, indicating that T cells from arthritic wild type mice recapitulate the motile behavior reported in naïve TCR-transgenic mice. However, naïve T cells also engaged in stable interactions with APCs in the JDLNs of arthritic recipients, suggesting an enhanced ability of APCs in the JDLNs of arthritic hosts to present antigen to either naïve or antigen-experienced T cells.
► We monitor activation-related T cell motility in vivo in genetically unaltered mice. ► We visualize T cells in joint-draining lymph nodes in an arthritis model. ► Antigen-experienced T cells show motility similar to that in TCR transgenic mice. ► T cells are preferentially arrested in joint-draining lymph nodes of arthritic mice. ► The motility of naïve T cells is influenced by cellular competition and inflammation.
