Inflammation-inducing Th1 and Th17 cells differ in their expression patterns of apoptosis-related molecules

Th1 cells are remarkably more susceptible to activation induced cell death than Th17. Here, we compared cultures of these two cell subpopulations for their expression of apoptosis-related molecules when re-exposed to their specific antigen. We also compared the expression of apoptosis-related molecules in the mouse eye with inflammation induced by Th1 or Th17 cells. Using qPCR we found that the mRNA transcript levels of the majority of tested apoptosis-related molecules were higher in the Th1 cultures, and in eyes with Th1-induced inflammation. Apoptotic intrinsic pathway molecules played minor roles in the processes in vitro or in vivo, whereas extrinsic pathway molecules, as well as PD-1, its ligands and Tim3, were heavily involved.

► Th1 cell cultures express apoptosis-related molecules more than Th17 cells do. ► Th1-induced inflammation highly involves apoptosis-related molecules. ► Extrinsic pathway of apoptosis participates in immune-mediated inflammation.