Antibody binding to porcine sialoadhesin reduces phagocytic capacity without affecting other macrophage effector functions

Sialoadhesin (Sn) is a macrophage-restricted endocytic receptor involved in cell–cell, cell–matrix and cell–pathogen interactions. Recently, porcine Sn (pSn) was shown to be involved in signaling and lately Sn is gaining interest as a potential target for immunotherapy. However, little is known about the effect of ligand binding to Sn on macrophage effector functions. In this study, we tested the effect of antibody binding to pSn on macrophage viability, phagocytosis of microspheres, uptake and processing of soluble antigens, reactive oxygen/nitrogen species production, MHC I and MHC II cell surface expression and cytokine production. This was done by treatment of porcine primary alveolar macrophages with the pSn-specific mAb 41D3, or an isotype-matched control mAb. No significant effect on most effector functions under study was observed, except for a significant reduction of phagocytosis. Thus, antibody binding to pSn can downregulate phagocytosis, which could have implications on homeostasis, infectious and immune diseases, and immunotherapy.

► Sn is a macrophage-restricted endocytic receptor and a target for immunotherapy. ► The effect of mAb binding to pSn on macrophage viability and functions was tested. ► Phagocytosis, OVA-DQ processing, ROI and cytokine production, MHC I/II expression. ► mAb binding to Sn causes a decrease in phagocytosis, not affecting other functions. ► Possible implications on homeostasis, infectious/immune diseases, immunotherapy.