Article ID | Journal | Published Year | Pages | File Type |
---|---|---|---|---|
2167780 | Cellular Immunology | 2009 | 6 Pages |
Abstract
Recent studies have implicated the cell surface receptor Programmed Death-1 (PD-1) in numerous models of T cell anergy, though the specific mechanisms by which the PD-1 signal maintains tolerance is not clear. We demonstrate that the depletion of PD-1 with siRNA results in a complete reversal of clonal anergy in the A.E7 T cell model, suggesting that the mechanism by which PD-1 maintains the anergic phenotype is a T-cell-intrinsic phenomenon, and not one dependent on other cell populations in vivo. We have also shown that the neutralization of IL-2 during restimulation abrogates the effect of PD-1 depletion, suggesting that tolerance mediated by PD-1 is wholly IL-2 dependent, and likewise intrinsic to the tolerized cells.
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Authors
Kenneth D. Bishop, John E. Harris, John P. Mordes, Dale L. Greiner, Aldo A. Rossini, Michael P. Czech, Nancy E. Phillips,