Coordination of molecular motors: from in vitro assays to intracellular dynamics

New technologies have emerged that enable the tracking of molecular motors and their cargos with very high resolution both in vitro and in live cells. Classic in vitro motility assays are being supplemented with assays of increasing complexity that more closely model the cellular environment. In cells, the introduction of probes such as quantum dots allows the high-resolution tracking of both motors and vesicular cargos. The ‘bottom up’ enhancement of in vitro assays and the ‘top down’ analysis of motility inside cells are likely to converge over the next few years. Together, these studies are providing new insights into the coordination of motors during intracellular transport.