| Article ID | Journal | Published Year | Pages | File Type |
|---|---|---|---|---|
| 2171985 | Cytotherapy | 2011 | 4 Pages |
Abstract
Adherent adipose-derived stromal/stem cells (ASC) have been used in pre-clinical regenerative medical studies applied to a broad range of tissues with an ultimate goal of translating these findings to clinical safety and efficacy testing; however, many protocols passage the cells using porcine-derived trypsin. We have compared porcine trypsin with animal protein-free products from recombinant bacteria (TrypLE Express; Invitrogene) and corn (TrypZean; Sigma) based on cell yield, viability and immunophenotype. ASC harvested with each trypsin product were comparable.
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Authors
Pedro P. Carvalho, Xiying Wu, Gang Yu, Marilyn Dietrich, Isabel R. Dias, Manuela E. Gomes, Rui L. Reis, Jeffrey M. Gimble,