Reduction of Cullin-2 in somatic cells disrupts differentiation of germline stem cells in the Drosophila ovary

•Cullin-2 (Cul-2) expresses in germline as well as somatic cells of Drosophila ovary.•Reduction of Cul-2 in somatic cells produces extra germline stem cell-like cells.•Those extra cells originate due to the elevation of Dpp signaling in the niche.•This report shows a cell non-autonomous regulation of Dpp signaling by Cul-2.

Signaling from a niche consisting of somatic cells is essential for maintenance of germline stem cells (GSCs) in the ovary of Drosophila. Decapentaplegic (Dpp), a type of bone morphogenetic protein (BMP) signal, emanating from the niche, is the most important signal for this process. Cullin proteins constitute the core of a multiprotein E3-ligase important for their functions viz. degradation or modification of proteins necessary for different cellular processes. We have found that a Cullin protein called Cullin-2 (Cul-2) expresses in both somatic and germline cells of the Drosophila ovary. Reduction of Cul-2 in somatic cells causes upregulation of Dpp signal and produces accumulation of extra GSC-like cells inside germarium, the anteriormost structure of the ovary. Our results suggest that Cullin-2 protein present in the somatic cells is involved in a non cell-autonomous regulation of the extent of Dpp signaling and thus controls the differentiation of GSCs to cystoblasts (CBs).