| Article ID | Journal | Published Year | Pages | File Type |
|---|---|---|---|---|
| 2176416 | Developmental Cell | 2015 | 10 Pages |
•Embryonic microglia in zebrafish initiate from the rostral blood island•Adult microglia in zebrafish arise from the ventral wall of dorsal aorta•The RBI- and VDA-derived microglia are differentially regulated by Pu.1•The VDA-derived microglia are Runx1 dependent but cMyb independent
SummaryMicroglia are CNS resident macrophages, and they play important roles in neural development and function. Recent studies have suggested that murine microglia arise from a single source, the yolk sac (YS), yet these studies lack spatial resolution to define the bona fide source(s) for microglia. Here, using light-induced high temporal-spatial resolution fate mapping, we challenge this single-source view by showing that microglia in zebrafish arise from multiple sources. The embryonic/larval microglia originate from the rostral blood island (RBI) region, the equivalent of mouse YS for myelopoiesis, whereas the adult microglia arise from the ventral wall of dorsal aorta (VDA) region, a tissue also producing definitive hematopoiesis in mouse. We further show that the VDA-region-derived microglia are Runx1 dependent, but cMyb independent, and developmentally regulated differently from the RBI region-derived microglia. Our study establishes a new paradigm for investigating the development and function of distinct microglia populations.
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