Genetic Control of Plant Development by Overriding a Geometric Division Rule

•A complete 4D map of early Arabidopsis embryo development•Volumetric asymmetry after division correlates with differential cell identity•A geometric rule connects 3D cell shape to oriented division•Auxin promotes asymmetric division by suppressing the geometric default rule

SummaryFormative cell divisions are critical for multicellular patterning. In the early plant embryo, such divisions follow from orienting the division plane. A major unanswered question is how division plane orientation is genetically controlled, and in particular whether this relates to cell geometry. We have generated a complete 4D map of early Arabidopsis embryogenesis and used computational analysis to demonstrate that several divisions follow a rule that uses the smallest wall area going through the center of the cell. In other cases, however, cell division clearly deviates from this rule, which invariably leads to asymmetric cell division. By analyzing mutant embryos and through targeted genetic perturbation, we show that response to the hormone auxin triggers a deviation from the “shortest wall” rule. Our work demonstrates that a simple default rule couples division orientation to cell geometry in the embryo and that genetic regulation can create patterns by overriding the default rule.

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